Generation of Self-assembled Vascularized Human Skin Equivalents

Tissue Engineered Human Skin Equivalents

Human skin not only serves as an important barrier against the penetration of exogenous substances into the body, but also provides a potential avenue for the transport of functional active drugs/reagents/ingredients into the skin (topical delivery) and/or the body (transdermal delivery). In the past three decades, research and development in human skin equivalents have advanced in parallel wit...

Development of culture media for the construction of vascularized adipose tissue and vascularized 3D full-skin equivalents in vitro

Generation of 3D Skin Equivalents Fully Reconstituted from Human Induced Pluripotent Stem Cells (iPSCs)

Recent generation of patient-specific induced pluripotent stem cells (PS-iPSCs) provides significant advantages for cell- and gene-based therapy. Establishment of iPSC-based therapy for skin diseases requires efficient methodology for differentiating iPSCs into both keratinocytes and fibroblasts, the major cellular components of the skin, as well as the reconstruction of skin structures using t...

Self organization of Fibroblasts and Keratinocytes in morphogenesis of dermal-epidermal junction in human skin equivalents

Introduction Dermal epidermal junction (DEJ) is a complex macromolecular structure which establishes the boundary between two major skin compartments, epidermis and dermis and the cellular components of these layers play a major role in its organization (1). We are interested in the structural and functional analysis of the epidermis formed in human skin equivalent (HSE) models that uses co-cul...

Bioengineered Self-assembled Skin as an Alternative to Skin Grafts

For patients with extensive burns or donor site scarring, the limited availability of autologous and the inevitable rejection of allogeneic skin drive the need for new alternatives. Existing engineered biologic and synthetic skin analogs serve as temporary coverage until sufficient autologous skin is available. Here we report successful engraftment of a self-assembled bilayered skin construct d...